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Basics & Technique

How do I reconstitute a 5 mg TB-500 vial?

Updated 2026-05-05

Add 2 mL of bacteriostatic water to a 5 mg TB-500 vial. That gives you 2.5 mg per mL. On a U-100 insulin syringe, 10 units equals 250 mcg, 20 units equals 500 mcg, and a full 100-unit syringe equals 2.5 mg. Most TB-500 protocols use 2.5 mg twice a week, so each shot is one full insulin syringe. Pour the water down the vial wall, swirl gently, and refrigerate.

IfIf your dose is 2.5 mg
Thenthen draw a full 100 units on a U-100 insulin syringe
IfIf your dose is 500 mcg
Thenthen draw 20 units on a U-100 insulin syringe
IfIf you want a smaller injection volume per shot
Thenthen reconstitute with 1 mL instead of 2 mL - that gives 5 mg/mL, so 2.5 mg = 50 units
IfIf you stack TB-500 with BPC-157
Thenthen keep the two vials separate and pull each into its own syringe - never mix them in one vial
Key facts
  • 5 mg vial + 2.0 mL bac water = 2.5 mg/mL concentration
  • Most protocols dose 2.5 mg twice per week for 4-6 weeks loading, then taper
  • TB-500 half-life is about 2-3 hours after a subcutaneous shot
  • No published human trials confirm dosing - protocols come from clinical practice and community use
  • Mixed vial keeps in the fridge ~28 days; do not freeze
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